The SHSYSY cells have been growing on silk fibres since 24th May 09. Today I removed the tangles of fibres with their attacehd cells and placed them into new wells with fresh media - just to see. Silky nerves, nervous silks. It’s all incredibly crude but I’m just following my nose.
Mel Grant introduced me to Eric Hill who has been working with neuron and astrocyte cells extensively. He’s made many spheres of neurons (known as blobs) by growing them on bacteria petri dishes, picking them up by pipette every two days so that they don’t attach and changing the medium - I think. I need to get the protocol but they grow in exquisite spheres rather than on the surface of the dish. Bacteria dishes are not tissue culture treated so not expecailly attractive to cells to attach to. This technique is called something like embryo spheres, Janet has done alot of it.